Advertisement

The effect of fibrin stabilizing factor (F.XIII) on healing of bone defects in normal and uncontrolled diabetic rats

  • Author Footnotes
    * Address: Dr Ibrahim E. El-Hakim 6 El-Gendy Street Hadayek Helwan 11433 Cairo Egypt Tel: +20 2 3690289
    Ibrahim E. El-Hakim
    Footnotes
    * Address: Dr Ibrahim E. El-Hakim 6 El-Gendy Street Hadayek Helwan 11433 Cairo Egypt Tel: +20 2 3690289
    Affiliations
    Faculty of Dental Medicine, Ain Shams University, Cairo, Egypt
    Search for articles by this author
  • Author Footnotes
    * Address: Dr Ibrahim E. El-Hakim 6 El-Gendy Street Hadayek Helwan 11433 Cairo Egypt Tel: +20 2 3690289
      This paper is only available as a PDF. To read, Please Download here.

      Abstract

      This study was undertaken to examine the effect of fibrin stabilizing factor (F.XIII) on healing of bone defects in normal and uncontrolled diabetic rats. Eighty rats were divided into two groups: group I (diabetic) and group II (non-diabetic) (40 rats each). Diabetes was induced in group I using streptozotocin. Both groups were divided into two subgroups, control and experimental (20 rats each). Bone defect was created in the mandible. Rats in the experimental subgroups were injected with F.XIII, while those of the control groups were injected with saline (F.XIII solvent). Animals were killed at varying intervals and tissue sections stained with hematoxyline and eosin and Van-Gieson stains were examined. Differences in collagen deposition and bone formation were compared in both control and experimental groups. Collagen deposition was evident and appeared more oriented in diabetic rats treated with F.XIII, and signs of bone deposition started in the experimental group earlier than in the control group. On the other hand, F.XIII did not significantly affect healing in non-diabetic rats. It is concluded from these results that F.XIII may enhance early stages of bone healing in uncontrolled diabetic rats.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to International Journal of Oral and Maxillofacial Surgery
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Abbey LM
        • Cohen M
        • Shklar G
        The effect of streptozotocin induced diabetes on the healing of artificially produced tongue wounds in rats.
        Oral Surg. 1972; 33: 672-683
        • Aeschlimann D
        • Mosher D
        • Paulsson M
        Tissue transglutaminase and factor XIII in cartilage and bone remodeling.
        Semin Thromb Hemost. 1996; 22: 437-443
        • Benfer J
        • Struck H
        Factor XIII and fracture healing, an experimental study.
        Eur Surg Res. 1977; 9: 217-223
        • Bennet RJ
        An experimental study of wound healing after pancreatectomy.
        in: Master's Thesis. University of Minnesota, 1932
        • Chamouard P
        • Grunebaum L
        • Wiesel ML
        • et al.
        Significance of diminished factor XIII in Crohn's disease.
        Am J Gastroenterol. 1998; 93: 610-614
        • Claes L
        • Burri C
        • Gerngross H
        • Mutschler W
        Bone healing stimulated by plasma factor XIII. Osteotomy experiments in sheep.
        Acta Orthop Scand. 1985; 56: 57-62
        • Devlin H
        • Garland H
        • Sloan P
        Healing of tooth extraction sockets in experimental diabetes mellitus.
        J Oral Maxillofac Surg. 1996; 54: 1087-1091
        • Eaglton WP
        Influence of old age, diabetes, arteriosclerosis and gout on the healing of the wounds.
        Am Med. 1904; 7: 898
        • Goodson WH
        • Hunt TK
        Wound healing in diabetic patients.
        Surg Gynecol Obstet. 1979; 149: 600-608
        • Hellerer O
        • Bruckner W
        • Westerburg K
        • Klessinger U
        Fracture healing under Factor XIII medication.
        Arch Orthop Trauma Surg. 1980; 97: 157-159
        • Hove K
        • Stallard R
        Diabetes and the periodontal patient.
        J Periodont. 1970; 41: 53-58
        • Letheby BA
        • Davis R
        • Larsen A
        The effect of major surgical procedures on plasma and platelet levels of Factor XIII.
        Thromb Diath Haemorrhag. 1974; 31: 20-23
        • Lien YH
        • Tseng MM
        • Stern R
        Glucose and glucose analogs modulate collagen metabolism.
        Exp Mol Pathol. 1992; 57: 215-219
        • Melcher AH
        Behaviour of collagen and reticulin in inflammation.
        in: Jackson SF Structure and inflammation of connective skeletal tissue. Butterworths, London1965: 500
        • Mordes J
        • Rossini A
        Animal model of diabetes.
        Am J Med. 1981; 70: 353-360
        • Murphy L
        • Lazarus L
        The effect of streptozotocin induced diabetes mellitus on the ability of rat serum to stimulate human fibroblast proliferation.
        Endocrinology. 1983; 112: 1026-1035
        • Nishigaki A
        Experimental studies of skin wound healing process by first intention in streptozotocin-induced diabetes mellitus rats.
        Shikwa Gakuho. 1989; 89: 793-822
        • Ronneberger VH
        • Hein B
        Untersuchungen mit einem Fibrin-Kleber an Rattenhaut-Stanzwunden.
        Arzneim Forsch. 1984; 34: 287-290
        • Seifter E
        • Rettura G
        • Padawer J
        • Stratford F
        • Kamboses D
        • Levenson S
        Impaired wound healing in streptozotocin diabetes, prevention by supplemental vitamin A.
        Ann Surg. 1981; 194: 42-50
        • Soria A
        • Soria C
        • Boulard C
        Fibrin stabilizing factor (F.XIII) and collagen polymerisation.
        Specialia. 1975; 15th November: 1355-1357
        • Spanheimer RG
        • Umpierrez GE
        • Stumpf V
        Decreased collagen production in diabetic rats.
        Diabetes. 1988; 37: 371-376
        • Weringer E
        • Kelso J
        • Tamai I
        • Arquilla E
        The effect of insulin on wound healing in diabetic mice.
        Acta Endocrinol. 1982; 99: 101-108