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Flow cytometric S-phase fraction contributes to diagnosis of diploid malignant salivary gland tumours

  • O. Driemel
    Affiliations
    Department of Oral and Maxillofacial Surgery, University of Regensburg, 89053 Regensburg, Germany
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  • K. Kraft
    Affiliations
    Department of Pathology, Military Hospital Ulm, 89070 Ulm, Germany
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  • J. Hemmer
    Correspondence
    Address: Joerg Hemmer, Department of Tumour Biology, University of Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany. Tel: +49 731 502 3754; Fax: +49 731 502 3758.
    Affiliations
    Department of Tumour Biology, University of Ulm, 89081 Ulm, Germany

    Department of Oral Pathology and Oral Biology, University of Pretoria, Pretoria 0001, South Africa
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      Abstract

      DNA ploidy studies on salivary gland tumours have shown that the proportion of aneuploid cases, although confined to the malignant entities, is considerably lower than for other solid malignancies. To analyse whether the S-phase fraction (SPF) may contribute to discrimination of diploid malignant from benign tumours, DNA flow cytometric data from 45 different malignant salivary gland tumours was compared with that of 121 pleomorphic adenomas. All benign tumours were diploid. Twelve malignant tumours contained aneuploid cell populations. The SPF values for diploid malignancies ranged between 0.9% and 11.0% (mean 3.9%), and between 0.5% and 7.9% (mean 2.7%) for pleomorphic adenomas. A 4% cut-off value gained statistical significance for discriminating diploid malignant tumours from pleomorphic adenomas (P < 0.01). The sensitivity for SPF > 4% was 46% and the positive predictive value was 40%. A sensitivity of 60% and a positive predictive value of 54% was achieved by combining aneuploidy and SPF > 4%. These results show that DNA flow cytometry may contribute to diagnostic assessment in salivary gland tumours.

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