Abstract
This study investigated the effect of resveratrol on bone healing and its influence
on the gene expression of osteogenic markers. Two calvarial defects were created and
one screw-shaped titanium implant was inserted in the tibia of rats that were assigned
to daily administration of placebo (control group, n = 15) or 10 mg/kg of resveratrol (RESV group, n = 15) for 30 days. The animals were then sacrificed. One of the calvarial defects was
processed for histomorphometric analysis and the tissue relative to the other was
collected for mRNA quantification of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin
(OPN), bone sialoprotein (BSP), osteoprotegrin (OPG), and receptor activator of NF-κB
ligand (RANKL). Implants were removed by applying a counter-torque force. Histomorphometric
analysis revealed higher remaining defect in the calvarial defects of the control
group than the RESV group (P = 0.026). Resveratrol increased the counter-torque values of implant removal when compared
to control therapy (P = 0.031). Gene expression analysis showed a higher expression of BMP-2 (P = 0.011), BMP-7 (P = 0.049), and OPN (P = 0.002) genes in the RESV group than in the control group. In conclusion, resveratrol
improved the repair of critical-sized bone defects and the biomechanical retention
of implants. Indeed, this natural agent may up-regulate the gene expression of important
osteogenic markers.
Key words
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References
- Selective cyclooxygenase-2 inhibitor may impair bone healing around titanium implants in rats.J Periodontol. 2006; 77: 1731-1735
- Factors associated with dental implant survival: a 4-year retrospective analysis.J Periodontol. 2011; 82: 1390-1395https://doi.org/10.1902/jop.2011.100685
- Fracture healing under healthy and inflammatory conditions.Nat Rev Rheumatol. 2012; 8: 133-143https://doi.org/10.1038/nrrheum.2012.1
- Bisphosphonate-related osteonecrosis of the jaw in patients with oral bisphosphonate exposure: clinical course and outcomes.J Oral Maxillofac Surg. 2012; 70: 1844-1853https://doi.org/10.1016/j.joms.2011.08.033
- Parathyroid hormone applications in the craniofacial skeleton.J Dent Res. 2013; 92: 18-25https://doi.org/10.1177/0022034512464779
- PTH administration promotes tooth extraction socket healing.J Dent Res. 2013; 92: 553-559https://doi.org/10.1177/0022034513487558
- PTH 1–84: bone rebuilding as a target for the therapy of severe osteoporosis.Clin Cases Miner Bone Metab. 2012; 9: 31-36
- Promotion of peri-implant bone healing by systemically administered parathyroid hormone (1–34) and zoledronic acid adsorbed onto the implant surface.Osteoporos Int. 2013; 24: 1063-1071https://doi.org/10.1007/s00198-012-2258-5
- Effect of parathyroid hormone (1–34) on fractures and bone mineral density in postmenopausal women with osteoporosis.N Engl J Med. 2001; 344: 1434-1441
- Capillary electrophoretic determination of resveratrol in wines.J Agric Food Chem. 1999; 47: 3223-3227
- Oral resveratrol reduces neuronal damage in a model of multiple sclerosis.J Neuroophthalmol. 2010; 30: 328-339https://doi.org/10.1097/WNO.0b013e3181f7f833
- Evaluation of renal protective effects of the green-tea (EGCG) and red grape resveratrol: role of oxidative stress and inflammatory cytokines.Nat Prod Res. 2011; 25: 850-856https://doi.org/10.1080/14786419.2010.533669
- Anti-inflammatory effect of resveratrol and polydatin by in vitro IL-17 modulation.Inflammation. 2012; 35: 240-248https://doi.org/10.1007/s10753-011-9310-z
- Resveratrol prevents RANKL-induced osteoclast differentiation of murine osteoclast progenitor RAW 264. 7 cells through inhibition of ROS production.Biochem Biophys Res Commun. 2010; 401: 356-362https://doi.org/10.1016/j.bbrc.2010.09.053
- Resveratrol promotes osteogenesis of human mesenchymal stem cells by upregulating RUNX2 gene expression via the SIRT1/FOXO3A axis.J Bone Miner Res. 2011; 26: 2552-2563https://doi.org/10.1002/jbmr.460
- Preventing bone loss and weight gain with combinations of vitamin D and phytochemicals.J Med Food. 2011; 14: 1352-1362https://doi.org/10.1089/jmf.2010.0232
- Effect of resveratrol on bone formation in the expanded inter-premaxillary suture: early bone changes.Orthod Craniofac Res. 2011; 14: 80-87https://doi.org/10.1111/j.1601-6343.2011.01511.x
- Resveratrol decreases periodontal breakdown and modulates local levels of cytokines during periodontitis in rats.J Periodontol. 2013; 84: e58-e64
- Repairing critical-sized calvarial defects with BMSCs modified by a constitutively active form of hypoxia-inducible factor-1α and a phosphate cement scaffold.Biomaterials. 2011; 32: 9707-9718https://doi.org/10.1016/j.biomaterials.2011.09.005
- Effect of aluminum oxide-blasted implant surface on the bone healing around implants in rats submitted to continuous administration of selective cyclooxygenase-2 inhibitors.Int J Oral Maxillofac Implants. 2009; 24: 226-233
- Biomechanical evaluation of macro and micro designed screw-type implants: an insertion torque and removal torque study in rabbits.Clin Oral Implants Res. 2013; 24: 342-346https://doi.org/10.1111/j.1600-0501.2011.02336.x
- Effects of induced diabetes and the administration of aminoguanidine in the biomechanical retention of implants: a study in rats.J Periodontal Res. 2011; 46: 691-696https://doi.org/10.1111/j.1600-0765.2011.01391.x
- Perinatal periodontal disease reduces social behavior in male offspring.Neuroimmunomodulation. 2013; 20: 29-38https://doi.org/10.1159/000342987
- Effects of trans-resveratrol from Polygonum cuspidatum on bone loss using the ovariectomized rat model.J Med Food. 2005; 8: 14-19
- Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in a rat calvarial defect model.J Periodontol. 2004; 75: 1364-1370
- Oxysterols enhance osteoblast differentiation in vitro and bone healing in vivo.J Orthop Res. 2007; 25: 1488-1497
- Alendronate enhances osseous healing in a rat calvarial defect model.Arch Oral Biol. 2012; 57: 1545-1550https://doi.org/10.1016/j.archoralbio.2012.06.013
- Effect of zoledronate acid treatment on osseointegration and fixation of implants in autologous iliac bone grafts in ovariectomized rabbits.Bone. 2012; 50: 119-127https://doi.org/10.1016/j.bone.2011.10.011
- Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy.Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 97: 693-700
- Effect of systemic parathyroid hormone (1–34) and a beta-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model.J Clin Periodontol. 2010; 37: 419-426https://doi.org/10.1111/j.1600-051X.2010.01547.x
- Effect of systemic intermittent administration of human parathyroid hormone (rhPTH[1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34]) on the resistance to reverse torque in rabbit tibiae.J Oral Implantol. 2008; 34: 298-302https://doi.org/10.1563/1548-1336-34.6.298
- The effect of systemically administrated zoledronic acid on the osseointegration of dental implants.Oral Dis. 2012; 18: 802-808https://doi.org/10.1111/j.1601-0825.2012.01948.x
- Forkhead proteins are critical for bone morphogenetic protein-2 regulation and anti-tumor activity of resveratrol.J Biol Chem. 2007; 282: 19385-19398
- BMP-6-induced osteogenic differentiation of mesenchymal cell lines is not modulated by sex steroids and resveratrol.Cytokine. 2003; 23: 133-137
- Expression of osteoblast marker genes in rat calvarial osteoblast-like cells, and effects of the endocrine disrupters diphenylolpropane, benzophenone-3, resveratrol and silymarin.Chem Biol Interact. 2006; 164: 147-156
- Resveratrol inhibits myeloma cell growth, prevents osteoclast formation, and promotes osteoblast differentiation.Cancer Res. 2005; 65: 9943-9952
- Resveratrol-mediated SIRT-1 interactions with p300 modulate receptor activator of NF-kappaB ligand (RANKL) activation of NF-kappaB signaling and inhibit osteoclastogenesis in bone-derived cells.J Biol Chem. 2011; 286: 11492-11505https://doi.org/10.1074/jbc.M110.198713
- Activation of Sirt1 decreases adipocyte formation during osteoblast differentiation of mesenchymal stem cells.J Bone Miner Res. 2006; 21: 993-1002
- Effect of catch-up growth by various dietary patterns and resveratrol intervention on bone status.Exp Biol Med (Maywood). 2012; 237: 297-304https://doi.org/10.1258/ebm.2011.011296
Article info
Publication history
Published online: February 18, 2014
Accepted:
January 13,
2014
Identification
Copyright
© 2014 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.
