The effect of platelet-rich plasma on early and late bone healing using a mixture of particulate autogenous cancellous bone and Bio-Oss^®: an experimental study in goats

The four experimental sites (defects). A and C filled with 0.3g cancellous bone and 0.2g Bio-Oss^® granules (1–2mm). B and D filled with 0.3g cancellous bone, 0.2g Bio-Oss^® granules (1–2mm) and 1ml PRP each.

Histomorphometric analysis of a PRP-treated 14mm defect, using Bio-Oss^® particles and autogenous bone particles, 2 weeks postoperatively. Staining: methylene blue and basic fuchsin. (A) The original section, which was digitized at low magnification, (B) the outline of the surface of the defect (blue dotted line) is depicted, (C) bony edges of the defect, autogenous bone particles, and newly formed bone are marked (green), (D) Bio-Oss^® particles are marked (green). Bone fill percentage and Bio-Oss^® fill percentage were calculated based on these observations. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)

Histological analysis of a PRP-treated 14mm defect, using Bio-Oss^® granules and autogenous bone particles, 6 weeks postoperatively. Bony edges of the defect, autogenous bone particles, newly formed bone (pink) and Bio-Oss^® particles (brown) are visible. There was less newly formed bone at the surface of the Bio-Oss^® particles compared with the autogenous bone particles. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)

Histomorphometrical calculations of the surface of the defect, concerning the PRP and control group, after 1, 2, 6, and 12 weeks postoperatively.

Histomorphometrical calculations of the bone fill percentage in the defect of the PRP and control group, after 1, 2, 6, and 12 weeks postoperatively.

Histomorphometrical calculations of the Bio-Oss^® fill percentage in the defect of the PRP and control group, after 1, 2, 6, and 12 weeks postoperatively.