Distribution of Small Integrin-Binding LIgand, N-linked Glycoproteins (SIBLING) in the condylar cartilage of rat mandible

Sun, Y.; Gandhi, V.; Prasad, M.; Yu, W.; Wang, X.; Zhu, Q.; Feng, J.Q.; Hinton, R.J.; Qin, C.

doi:10.1016/j.ijom.2009.12.017

« Previous Next »International Journal of Oral & Maxillofacial Surgery
Volume 39, Issue 3 , Pages 272-281, March 2010

Distribution of Small Integrin-Binding LIgand, N-linked Glycoproteins (SIBLING) in the condylar cartilage of rat mandible

Y. Sun
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
- Department of Oral and Maxillofacial Surgery, The 2nd Affiliated Hospital of Harbin Medical University, Heilongjiang, China
V. Gandhi
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
M. Prasad
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
W. Yu
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
X. Wang
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
Q. Zhu
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
J.Q. Feng
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
R.J. Hinton
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
C. Qin
- Department of Biomedical Sciences, Baylor College of Dentistry, Texas A & M University System Health Science Center, Dallas, TX, USA
- Address: Chunlin Qin, Department of Biomedical Sciences, Baylor College of Dentistry, 3302 Gaston Avenue-Room 400, Dallas, TX 75246, USA. Tel.: +1 214 828 8292; fax: +1 214 874 4538.

Accepted 22 December 2009. published online 25 January 2010.

Abstract

The Small Integrin-Binding LIgand, N-linked Glycoprotein (SIBLING) family is one category of non-collagenous proteins closely related to osteogenesis. In this study, the authors systematically evaluated the presence and distribution of four SIBLING family members, dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), bone sialoprotein (BSP) and osteopontin (OPN), in rat mandibular condylar cartilage using protein chemistry and immunohistochemistry. For protein chemistry, SIBLING proteins in the dissected condylar cartilage were extracted with 4M guanidium–HCl, separated by ion-exchange chromatography, and analyzed by Western immunoblotting. Immunohistochemistry was employed to assess the distribution of these four SIBLING proteins in the condylar cartilage of 2-, 5- and 8-week-old rats. Results from both approaches showed that all four members are expressed in the condylar cartilage. DSPP, unlike that observed in dentin and bone, exists as a full-length form (uncleaved) in the condylar cartilage. The NH₂-terminal fragment of DMP1 is mainly detected in the matrix of the cartilage while the COOH-terminal fragment is primarily localized in the nuclei of cells in the chondroblastic and hypertrophic layers. The data obtained in this investigation provide clues about the potential roles of these SIBLING proteins in chondrogenesis.